Tissue Culture of Fish Cell Lines
This article is about Tissue Culture of Fish Cell Lines. This is for the use of fish health personnel as guidelines for effective poikilothermic cell culture techniques. Method in cell culture is important in quality control used by National Wild Fish Health Survey. This article explains exact cell lines to make use for various fish species, the procedure needed for best growth of cell lines, standardization among laboratories, and a successful procedure for tissue culture.Each cell line cultured is required to have a back up through frozen storages. Cell culture system need constant care, sufficient nutrition, a suitable environment, and consistent checkups. The culture should be provided with most favorable environment for survival by a fish health biologist. The cell can be undesirable for viral testing of free ranging fish population if a favorable environment is not provided.
It is outlined in this article the recommended cell line and incubation temperature to able to identify the target viruses for various species.
The following are the maintenance materials of stock cell lines and passage of cell monolayers:
fish cell line of choice, L-glutamine, minimum essential medium with fetal bovine serum, trypsin, culture flask, pipettes and sterile cotton plugged, isoproponol, granulate cylinder, glass bottle, beaker, and bleach. In selecting and examining cell cultures it is recommended to use only one type of cell line culture at a time and to make sure that the working area is sterile to avoid contamination. Also make sure that the culture medium is visually clear and free of microbial or fungal infection. The occurrence of contaminated lines gives danger to the condition of the all cell lines. With the use of a tissue culture microscope the exact cell morphology concur of the description of cell line can be established. The cell culture should have no indication of infection and contamination. Cells that are young are then used in the stationary phase.
Like any other culturing procedure it is important to maintain a sterile workplace. This is to avoid contaminating the cultures. The success of culturing is in the sterility of the materials and surroundings. Brief discussion on the steps how to prepare a cell suspension and how to determine the appropriate medium volume and split ratio is also described in this article. Instruments used in determining the cell number in cell suspension is attained by the use of hemocytometer and a tissue culture microscope. Both the hemocytometer and the tissue culture microscope are used to accurately tally the number of cells.
Quality control in tissue culture is also discussed in this article. Quality control involves the principles on keeping a good quality of fish cell lines and preventing tissue culture toxins. The Standardization of cell lines for preliminary viral testing is a very important element of the Wild Fish Health Survey. In addition to the quality control of tissue culture is the screening of cell line. This is required with all fish diagnostic. From a complete set of instruments; hemocytometer and the tissue culture microscope and the proper environment ensures an accurate and successful procedure in culturing.
